Journal: ACS Measurement Science Au

Article Title: Luciferase Calibrants Enable Absolute Quantitation of Bioluminescence Power

doi: 10.1021/acsmeasuresciau.3c00036

Figure Lengend Snippet: Luciferase calibrant solutions. This chart details the measurements of a dilution series of luminescent calibrant solutions with quantified energy output. Nluc reactions contain 20 μM furimazine, and Fluc reactions contain 1.05 mM Bright-Glo, both in TBS pH 7.4 supplemented with 0.01% acetylated BSA. Energy outputs for 200 μL solutions describe their light levels between 1 and 2 min after initiating the bioluminescent reaction. Light emission data represent the mean value with percent standard deviations of <4 and ≤3% for Nluc and Fluc, respectively. Supporting Information includes a more detailed table with the mean values and their associated standard deviations (Figure S4).

Article Snippet: Nluc (Promega E499A) was diluted in TBS (50 mM Tris pH 7.4 + 150 mM NaCl) + 0.1 mg/mL acetylated BSA (Promega R3961) to generate a 2× of a 3-fold serial dilution (3000–1.372 pM).

Techniques: Luciferase